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neb buffers composition  (New England Biolabs)


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    Structured Review

    New England Biolabs neb buffers composition
    Neb Buffers Composition, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neb+buffers+composition/NEBuffer+Set/pmc12120656__ao4c11282_si_001-49-214-214
    Average 94 stars, based on 12 article reviews
    neb buffers composition - by Bioz Stars, 2026-09
    94/100 stars

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    Sequencing:

    Article Title: Fusions of Catalytically Inactive RusA to FokI Nuclease Coupled with PNA Enable Programmable Site-Specific Double-Stranded DNA Breaks
    Article Snippet: Strand Strand Sequence 5’ to 3’ S1 AGCTGATCGTACGATGCTAGTCGTCCATCAGGCTAGTCGATGCTAGCTGA CGTAGCTGATCGTAGCATCGTGCAGCTGATCGTAGCATCCGAATCA S2 ACGATGCTCGATCGATGCATGCTAGTCGACGTCGATCGTACGATGCTAGT CGATGCTAGCTGAGCTGCTAGGACGACTAGCATCGTACGATCAGCT S3 GCTAGCTAGTCGATCGTAGCATATCCTAGCAGCTCAGCTAGCATCGACTA GCATCGTACGATCGACGTCGACTAGCATGCATCGATCGAGCATCGT S4 TGATTCGGATGCTACGATCAGCTGCACGATGCTACGATCAGCTACGTCAG CTAGCATCGACTAGCCTGATGGATATGCTACGATCGACTAGCTAGC Table S5: Top and bottom oligos used to clone the target sequences in pUC19 vectors Oligo name Oligo sequence (5’---3’) 151-γPNA1-top with NdeI TACACAGGGTGGTCACGAGGGTGGGCTGACTGTACAACA TCCGCATTGAGAACCTCCCTTGAG 151-γPNA1-bottom with NdeI TACTCAAGGGAGGTTCTCAATGCGGATGTTGTACAGTCA GCCCACCCTCGTGACCACCCTGTG Table S6: Primers used in the current study Primer name Prime Sequence (5’ --- 3’) Employed for pET28 seq F GCATGACTGGTGGACAGCAA Sanger sequencing of pET28A-RusA expression clones pET28 seq R AGAGGCCCCAAGGGGTTATG 151-pUC19-PNA1-F TGTCTGTAAGCGGATGCCGG All the pUC19 target plasmids confirmation by Sanger sequencing, PCR amplification of pUC19 targets for producing the 151-pUC19-PNA1-R GCCAGCTGGCGTAATAGCG Table S7: Compositions of buffers used in this study Buffer Composition 1X NEB rCutSmart 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r1.1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.0@25°C NEB r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C HEPES Buffer 1M HEPES pH 7.5 MOPS Buffer 20 mM MOPS 5 mM CH3COONa 1 mM EDTA pH 7.0 PBS Buffer 137 mM NaCl 2.7 mM KCl 10 mM Na2HPO4 1.8 mM KH2PO4 pH 7.4 NEB Buffers composition adapted from (https://www.bioke.com/webshop/neb/b7030.html)

    Expressing:

    Article Title: Fusions of Catalytically Inactive RusA to FokI Nuclease Coupled with PNA Enable Programmable Site-Specific Double-Stranded DNA Breaks
    Article Snippet: Strand Strand Sequence 5’ to 3’ S1 AGCTGATCGTACGATGCTAGTCGTCCATCAGGCTAGTCGATGCTAGCTGA CGTAGCTGATCGTAGCATCGTGCAGCTGATCGTAGCATCCGAATCA S2 ACGATGCTCGATCGATGCATGCTAGTCGACGTCGATCGTACGATGCTAGT CGATGCTAGCTGAGCTGCTAGGACGACTAGCATCGTACGATCAGCT S3 GCTAGCTAGTCGATCGTAGCATATCCTAGCAGCTCAGCTAGCATCGACTA GCATCGTACGATCGACGTCGACTAGCATGCATCGATCGAGCATCGT S4 TGATTCGGATGCTACGATCAGCTGCACGATGCTACGATCAGCTACGTCAG CTAGCATCGACTAGCCTGATGGATATGCTACGATCGACTAGCTAGC Table S5: Top and bottom oligos used to clone the target sequences in pUC19 vectors Oligo name Oligo sequence (5’---3’) 151-γPNA1-top with NdeI TACACAGGGTGGTCACGAGGGTGGGCTGACTGTACAACA TCCGCATTGAGAACCTCCCTTGAG 151-γPNA1-bottom with NdeI TACTCAAGGGAGGTTCTCAATGCGGATGTTGTACAGTCA GCCCACCCTCGTGACCACCCTGTG Table S6: Primers used in the current study Primer name Prime Sequence (5’ --- 3’) Employed for pET28 seq F GCATGACTGGTGGACAGCAA Sanger sequencing of pET28A-RusA expression clones pET28 seq R AGAGGCCCCAAGGGGTTATG 151-pUC19-PNA1-F TGTCTGTAAGCGGATGCCGG All the pUC19 target plasmids confirmation by Sanger sequencing, PCR amplification of pUC19 targets for producing the 151-pUC19-PNA1-R GCCAGCTGGCGTAATAGCG Table S7: Compositions of buffers used in this study Buffer Composition 1X NEB rCutSmart 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r1.1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.0@25°C NEB r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C HEPES Buffer 1M HEPES pH 7.5 MOPS Buffer 20 mM MOPS 5 mM CH3COONa 1 mM EDTA pH 7.0 PBS Buffer 137 mM NaCl 2.7 mM KCl 10 mM Na2HPO4 1.8 mM KH2PO4 pH 7.4 NEB Buffers composition adapted from (https://www.bioke.com/webshop/neb/b7030.html)

    Clone Assay:

    Article Title: Fusions of Catalytically Inactive RusA to FokI Nuclease Coupled with PNA Enable Programmable Site-Specific Double-Stranded DNA Breaks
    Article Snippet: Strand Strand Sequence 5’ to 3’ S1 AGCTGATCGTACGATGCTAGTCGTCCATCAGGCTAGTCGATGCTAGCTGA CGTAGCTGATCGTAGCATCGTGCAGCTGATCGTAGCATCCGAATCA S2 ACGATGCTCGATCGATGCATGCTAGTCGACGTCGATCGTACGATGCTAGT CGATGCTAGCTGAGCTGCTAGGACGACTAGCATCGTACGATCAGCT S3 GCTAGCTAGTCGATCGTAGCATATCCTAGCAGCTCAGCTAGCATCGACTA GCATCGTACGATCGACGTCGACTAGCATGCATCGATCGAGCATCGT S4 TGATTCGGATGCTACGATCAGCTGCACGATGCTACGATCAGCTACGTCAG CTAGCATCGACTAGCCTGATGGATATGCTACGATCGACTAGCTAGC Table S5: Top and bottom oligos used to clone the target sequences in pUC19 vectors Oligo name Oligo sequence (5’---3’) 151-γPNA1-top with NdeI TACACAGGGTGGTCACGAGGGTGGGCTGACTGTACAACA TCCGCATTGAGAACCTCCCTTGAG 151-γPNA1-bottom with NdeI TACTCAAGGGAGGTTCTCAATGCGGATGTTGTACAGTCA GCCCACCCTCGTGACCACCCTGTG Table S6: Primers used in the current study Primer name Prime Sequence (5’ --- 3’) Employed for pET28 seq F GCATGACTGGTGGACAGCAA Sanger sequencing of pET28A-RusA expression clones pET28 seq R AGAGGCCCCAAGGGGTTATG 151-pUC19-PNA1-F TGTCTGTAAGCGGATGCCGG All the pUC19 target plasmids confirmation by Sanger sequencing, PCR amplification of pUC19 targets for producing the 151-pUC19-PNA1-R GCCAGCTGGCGTAATAGCG Table S7: Compositions of buffers used in this study Buffer Composition 1X NEB rCutSmart 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r1.1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.0@25°C NEB r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C HEPES Buffer 1M HEPES pH 7.5 MOPS Buffer 20 mM MOPS 5 mM CH3COONa 1 mM EDTA pH 7.0 PBS Buffer 137 mM NaCl 2.7 mM KCl 10 mM Na2HPO4 1.8 mM KH2PO4 pH 7.4 NEB Buffers composition adapted from (https://www.bioke.com/webshop/neb/b7030.html)

    Polymerase Chain Reaction:

    Article Title: Fusions of Catalytically Inactive RusA to FokI Nuclease Coupled with PNA Enable Programmable Site-Specific Double-Stranded DNA Breaks
    Article Snippet: Strand Strand Sequence 5’ to 3’ S1 AGCTGATCGTACGATGCTAGTCGTCCATCAGGCTAGTCGATGCTAGCTGA CGTAGCTGATCGTAGCATCGTGCAGCTGATCGTAGCATCCGAATCA S2 ACGATGCTCGATCGATGCATGCTAGTCGACGTCGATCGTACGATGCTAGT CGATGCTAGCTGAGCTGCTAGGACGACTAGCATCGTACGATCAGCT S3 GCTAGCTAGTCGATCGTAGCATATCCTAGCAGCTCAGCTAGCATCGACTA GCATCGTACGATCGACGTCGACTAGCATGCATCGATCGAGCATCGT S4 TGATTCGGATGCTACGATCAGCTGCACGATGCTACGATCAGCTACGTCAG CTAGCATCGACTAGCCTGATGGATATGCTACGATCGACTAGCTAGC Table S5: Top and bottom oligos used to clone the target sequences in pUC19 vectors Oligo name Oligo sequence (5’---3’) 151-γPNA1-top with NdeI TACACAGGGTGGTCACGAGGGTGGGCTGACTGTACAACA TCCGCATTGAGAACCTCCCTTGAG 151-γPNA1-bottom with NdeI TACTCAAGGGAGGTTCTCAATGCGGATGTTGTACAGTCA GCCCACCCTCGTGACCACCCTGTG Table S6: Primers used in the current study Primer name Prime Sequence (5’ --- 3’) Employed for pET28 seq F GCATGACTGGTGGACAGCAA Sanger sequencing of pET28A-RusA expression clones pET28 seq R AGAGGCCCCAAGGGGTTATG 151-pUC19-PNA1-F TGTCTGTAAGCGGATGCCGG All the pUC19 target plasmids confirmation by Sanger sequencing, PCR amplification of pUC19 targets for producing the 151-pUC19-PNA1-R GCCAGCTGGCGTAATAGCG Table S7: Compositions of buffers used in this study Buffer Composition 1X NEB rCutSmart 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r1.1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.0@25°C NEB r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C HEPES Buffer 1M HEPES pH 7.5 MOPS Buffer 20 mM MOPS 5 mM CH3COONa 1 mM EDTA pH 7.0 PBS Buffer 137 mM NaCl 2.7 mM KCl 10 mM Na2HPO4 1.8 mM KH2PO4 pH 7.4 NEB Buffers composition adapted from (https://www.bioke.com/webshop/neb/b7030.html)

    Amplification:

    Article Title: Fusions of Catalytically Inactive RusA to FokI Nuclease Coupled with PNA Enable Programmable Site-Specific Double-Stranded DNA Breaks
    Article Snippet: Strand Strand Sequence 5’ to 3’ S1 AGCTGATCGTACGATGCTAGTCGTCCATCAGGCTAGTCGATGCTAGCTGA CGTAGCTGATCGTAGCATCGTGCAGCTGATCGTAGCATCCGAATCA S2 ACGATGCTCGATCGATGCATGCTAGTCGACGTCGATCGTACGATGCTAGT CGATGCTAGCTGAGCTGCTAGGACGACTAGCATCGTACGATCAGCT S3 GCTAGCTAGTCGATCGTAGCATATCCTAGCAGCTCAGCTAGCATCGACTA GCATCGTACGATCGACGTCGACTAGCATGCATCGATCGAGCATCGT S4 TGATTCGGATGCTACGATCAGCTGCACGATGCTACGATCAGCTACGTCAG CTAGCATCGACTAGCCTGATGGATATGCTACGATCGACTAGCTAGC Table S5: Top and bottom oligos used to clone the target sequences in pUC19 vectors Oligo name Oligo sequence (5’---3’) 151-γPNA1-top with NdeI TACACAGGGTGGTCACGAGGGTGGGCTGACTGTACAACA TCCGCATTGAGAACCTCCCTTGAG 151-γPNA1-bottom with NdeI TACTCAAGGGAGGTTCTCAATGCGGATGTTGTACAGTCA GCCCACCCTCGTGACCACCCTGTG Table S6: Primers used in the current study Primer name Prime Sequence (5’ --- 3’) Employed for pET28 seq F GCATGACTGGTGGACAGCAA Sanger sequencing of pET28A-RusA expression clones pET28 seq R AGAGGCCCCAAGGGGTTATG 151-pUC19-PNA1-F TGTCTGTAAGCGGATGCCGG All the pUC19 target plasmids confirmation by Sanger sequencing, PCR amplification of pUC19 targets for producing the 151-pUC19-PNA1-R GCCAGCTGGCGTAATAGCG Table S7: Compositions of buffers used in this study Buffer Composition 1X NEB rCutSmart 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r1.1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.0@25°C NEB r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C HEPES Buffer 1M HEPES pH 7.5 MOPS Buffer 20 mM MOPS 5 mM CH3COONa 1 mM EDTA pH 7.0 PBS Buffer 137 mM NaCl 2.7 mM KCl 10 mM Na2HPO4 1.8 mM KH2PO4 pH 7.4 NEB Buffers composition adapted from (https://www.bioke.com/webshop/neb/b7030.html)

    Recombinant:

    Article Title: Fusions of Catalytically Inactive RusA to FokI Nuclease Coupled with PNA Enable Programmable Site-Specific Double-Stranded DNA Breaks
    Article Snippet: Strand Strand Sequence 5’ to 3’ S1 AGCTGATCGTACGATGCTAGTCGTCCATCAGGCTAGTCGATGCTAGCTGA CGTAGCTGATCGTAGCATCGTGCAGCTGATCGTAGCATCCGAATCA S2 ACGATGCTCGATCGATGCATGCTAGTCGACGTCGATCGTACGATGCTAGT CGATGCTAGCTGAGCTGCTAGGACGACTAGCATCGTACGATCAGCT S3 GCTAGCTAGTCGATCGTAGCATATCCTAGCAGCTCAGCTAGCATCGACTA GCATCGTACGATCGACGTCGACTAGCATGCATCGATCGAGCATCGT S4 TGATTCGGATGCTACGATCAGCTGCACGATGCTACGATCAGCTACGTCAG CTAGCATCGACTAGCCTGATGGATATGCTACGATCGACTAGCTAGC Table S5: Top and bottom oligos used to clone the target sequences in pUC19 vectors Oligo name Oligo sequence (5’---3’) 151-γPNA1-top with NdeI TACACAGGGTGGTCACGAGGGTGGGCTGACTGTACAACA TCCGCATTGAGAACCTCCCTTGAG 151-γPNA1-bottom with NdeI TACTCAAGGGAGGTTCTCAATGCGGATGTTGTACAGTCA GCCCACCCTCGTGACCACCCTGTG Table S6: Primers used in the current study Primer name Prime Sequence (5’ --- 3’) Employed for pET28 seq F GCATGACTGGTGGACAGCAA Sanger sequencing of pET28A-RusA expression clones pET28 seq R AGAGGCCCCAAGGGGTTATG 151-pUC19-PNA1-F TGTCTGTAAGCGGATGCCGG All the pUC19 target plasmids confirmation by Sanger sequencing, PCR amplification of pUC19 targets for producing the 151-pUC19-PNA1-R GCCAGCTGGCGTAATAGCG Table S7: Compositions of buffers used in this study Buffer Composition 1X NEB rCutSmart 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r1.1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.0@25°C NEB r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C NEB r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 μg/ml Recombinant Albumin pH 7.9@25°C HEPES Buffer 1M HEPES pH 7.5 MOPS Buffer 20 mM MOPS 5 mM CH3COONa 1 mM EDTA pH 7.0 PBS Buffer 137 mM NaCl 2.7 mM KCl 10 mM Na2HPO4 1.8 mM KH2PO4 pH 7.4 NEB Buffers composition adapted from (https://www.bioke.com/webshop/neb/b7030.html)



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    neb buffers composition  (New England Biolabs)
    94
    New England Biolabs neb buffers composition
    Neb Buffers Composition, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neb+buffers+composition/NEBuffer+Set/pmc12120656__ao4c11282_si_001-49-214-214
    Average 94 stars, based on 1 article reviews
    neb buffers composition - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

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